NFKB1 Knockout cell line (U-87 MG)

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NFKB1 Knockout cell line (U-87 MG) Order

NFKB1 Knockout cell line (U-87 MG)
Catalog#
YKO-H770
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NFKB1 Knockout cell line (U-87 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NFKB1 Knockout cell line（U-87 MG）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
NFKB1
Gene ID
4790
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E1EK(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 6 (based on transcript NFKB1-201)
Validation: PCR+Sanger Sequencing
NFKB1 Knockout cell line (U-87 MG) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:U-87 MG		 STR Info (Cell bank)
Cell Line:U-87MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 811811
D7S820 8989
D16S539 12121212
VWA 15171517
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 10111011
D12S391 1821
FGA 1824
D2S1338 2023
D21S11 2832.2
D18S51 1313
D8S1179 1011
D3S1358 1617
D6S1043 1118
PENTAE 714
D19S433 1515.2
PENTAD 914
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NFKB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-87 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NFKB1 gene knockout NFKB1 Knockout cell line（U-87 MG） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NFKB1 Knockout cell line (U-87 MG)
NFKB1 Knockout cell line (U-87 MG)
Catalog#
YKO-H770
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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